Lyophylization of Produced Strains of the Rabies Virus

The parameters of lyophilization of substances of organ-tissue and cultural origin containing a fixed rabies virus were determined. The objects of this research were the “Moscow 3253,” СVS, and “Saratov” strains of the rabies virus used in the production of commercial and experimental batches of rabies immunoglobulin from horse blood serum. For substances containing “Moscow 3253,” CVS, and “Saratov” strains, the effects of both freezing and eutectic temperatures were identical. The use of the developed lyophilization protocol allowed us to obtain samples as well-formed porous tablets. The infectivity of the freeze-dried Moscow 3253, CVS, and Saratov strains was 6.2, 7.1, and 6.8 logID50/mL, respectively. Thus, the proposed freeze-drying regime is acceptable for obtaining samples of rabies virus strains used for the production of rabies immunoglobulin.