Sensitive detection of plasma interferon regulatory factor-5 (IRF5) by solid-phase proximity ligation assay validates IRF5 high and low subgroups in patients with systemic lupus erythematosus
The SP-PLA enabled sensitive and specific detection of low-level IRF5 in plasma and confirmed the presence of IRF5 high and low subgroups in SLE using a quantitative method. These findings support the potential of IRF5 as a biomarker, but validation in independent cohorts is required. The assay may facilitate patient stratification in future research and precision medicine approaches targeting the interferon pathway.
Authors
- S. Gräslund (ORCID: https://orcid.org/0000-0003-0077-8333)
- Charlotta Preger (ORCID: https://orcid.org/0000-0002-1342-9135)
- Iva Gunnarsson (ORCID: https://orcid.org/0000-0002-4514-7706)
- R Gallini (ORCID: https://orcid.org/0000-0002-2709-3337)
- Elisabet Svenungsson (ORCID: https://orcid.org/0000-0003-3396-3244)
- Helena Idborg (ORCID: https://orcid.org/0000-0003-4041-4729)
- Edvard Wigren (ORCID: https://orcid.org/0000-0002-7933-0939)
- Najibe Karami (ORCID: https://orcid.org/0009-0001-0836-1455)
Institutions
- Uppsala University (SE)
- Karolinska University Hospital (SE)
- Science for Life Laboratory (SE)
- Structural Genomics Consortium (CA)
- Karolinska Institutet (SE)
Publication Details
- Journal
- Scandinavian Journal of Rheumatology
- Published
- 2025-12-09
- DOI
- https://doi.org/10.1080/03009742.2025.2582936
- Primary Topic
- Systemic Lupus Erythematosus Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00