Lux-Biosensors for Analyzing the Intracellular Pool of Macroergic Compounds and Reducing Equivalents

At present, biosensor tools for analyzing intracellular energy processes are actively being developed. In this work, a set of plasmid constructs with luciferase genes from Luciola mingrelica and Photorhabdus luminescens was proposed for obtaining biosensor cells based on bacteria and eukaryotes. The possibility of targeted expression of luciferase both in the cytoplasm and in the mitochondrial matrix was realized due to the use of signal sequences. It was demonstrated that the resulting biosensors are suitable for analyzing the cell pool of macromolecular compounds and reducing equivalents, such as ATP, NADH, and flavin mononucleotide (FMN). We hope that this tool will be in demand for the analysis of the activity of intracellular chaperones, as well as toxicological and other investigations.