A novel polysaccharide from <i>Pholiota nameko</i> (T. Itô) S. Ito & S. Imai residue: chemical structure and hypoglycaemic activity <i>in vitro</i>
A novel polysaccharide (APNP) with a molecular weight of 3.56 × 10<sup>3</sup> KDa and a total sugar content of 92.35 ± 0.07% was isolated from <i>Pholiota nameko</i> (T. Itô) S. Ito & S. Imai residue using alkaline extraction. Monosaccharide composition analysis revealed that APNP consisted of galactose, glucose, and mannose. FT-IR and NMR analysis results indicate that APNP form linkages between (1→3), (1→6), and (1→3,6)-β-D-glucose, (1→)-α-D-glucose, (1→)-α-D-galactose, and (1→3,6)-β-D-mannose. Thermogravimetric analysis revealed three distinct pyrolysis phases in APNP. Congo Red assay results indicated the absence of triple-helical conformation in APNP under weakly alkaline conditions. <i>In vitro</i> assays demonstrated α-glucosidase inhibitory activity of APNP, with an IC<sub>50</sub> value of 0.165 mg/mL. HepG2-IR insulin resistance model and Western blot analyses demonstrated APNP's ability to ameliorate insulin resistance and enhance glucose metabolism.
Authors
- Liuya Wang
- X.Y. Zhang
- Tengda Li (ORCID: https://orcid.org/0000-0003-4411-7633)
- Chenxi Cui
- Lijing Chen (ORCID: https://orcid.org/0000-0002-2489-9933)
- Miao Wang (ORCID: https://orcid.org/0000-0002-2661-3011)
- Xueqing Geng (ORCID: https://orcid.org/0000-0003-2999-7396)
- Xiaojian Wu (ORCID: https://orcid.org/0000-0002-6275-0144)
- Huiqing Sun (ORCID: https://orcid.org/0009-0000-9292-6115)
- Zhen‐Yuan Zhu (ORCID: https://orcid.org/0000-0001-8016-5240)
Institutions
- Tianjin University of Science and Technology (CN)
- Guangxi Academy of Agricultural Science (CN)
- Huainan Normal University (CN)
Publication Details
- Journal
- Natural Product Research
- Published
- 2025-12-09
- DOI
- https://doi.org/10.1080/14786419.2025.2598059
- Primary Topic
- Polysaccharides and Plant Cell Walls
- Type
- article
- Field-Weighted Citation Impact
- 0.00