Warm-tolerant shiitake cultivation on alternative subtropical substrates

This study confirms that the suspected heat-tolerant L. edodes strains can be cultivated at 25°C, using alternative substrates such as Bursera, though substrate choice significantly affects productivity. Strain IE-3013 performed best, positioning it as a promising candidate for warm-climate cultivation. Cold-water immersion successfully induced the development of fruiting bodies across studied conditions, supporting its use in the shiitake cultivation process.