Varietal Identification of Lupine (Lupinus L.) Based on Microsatellite DNA Markers

Genetic polymorphism of a collection of accessions of the genus Lupinus was studied using SSR marking technology. The average level of intraspecies DNA polymorphism for L. albus, L. angustifolius, and L. luteus was found to be 25.8, 21.6, and 34.7%, respectively. The informative markers for genotyping and identification of Russian lupine varieties and breeding samples were determined. As a result of assessment of the genetic distance, genetically divergent accessions were identified that can serve as promising material for use in breeding programs for white (L. albus) and yellow (L. luteus) lupine, namely, SN 8-12, SN 96-15 DT1, KCI-22 d-15, KCI 22 d-17, 19-PIO-18-3, and SN 12-11-02-2-4-1. Based on the data obtained, molecular formulas of the studied samples were developed and reference genetic passports were compiled for the lupine varieties Belorozovyi 144, Nadyezhnyi, Antei, and Kurshavel. The practical results of this research and new knowledge on the structure of the genomes of Russian lupine varieties can be used in variety testing, seed production, and as a system of information support for the processes of selection and preservation of the national gene pool of lupine, a valuable agricultural crop.