FACED 2.0 enables large-scale voltage and calcium imaging in vivo
Monitoring neuronal activity at large scale and high spatiotemporal resolution is crucial for understanding information processing within the brain. Here we optimized a kilohertz-frame-rate two-photon fluorescence microscope with an all-optical megahertz line-scan rate to achieve ultrafast imaging across large areas and volumes at subcellular resolution. Applying this technique to in vivo voltage and calcium imaging, we demonstrated simultaneous recording of voltage activity over 200 neurons and calcium activity over 14,000 neurons from the mouse visual cortex, as well as volumetric calcium imaging of the larval zebrafish brain.
Authors
- Brent Doiron (ORCID: https://orcid.org/0000-0002-6916-5511)
- Kevin K. Tsia (ORCID: https://orcid.org/0000-0002-6394-9657)
- Xiaoyu Lu (ORCID: https://orcid.org/0000-0003-2426-1427)
- Christoph Miehl (ORCID: https://orcid.org/0000-0001-9094-2760)
- François St-Pierre (ORCID: https://orcid.org/0000-0001-8618-4135)
- Ryan G. Natan (ORCID: https://orcid.org/0000-0002-3123-4833)
- Na Ji (ORCID: https://orcid.org/0000-0002-5527-1663)
- Justin S. J. Wong (ORCID: https://orcid.org/0000-0002-4378-1199)
- K. Subhash C. Bose
- Qinrong Zhang (ORCID: https://orcid.org/0000-0001-8893-514X)
- Su Guo (ORCID: https://orcid.org/0000-0002-7342-0108)
- Jian Zhong (ORCID: https://orcid.org/0000-0003-1026-8695)
Institutions
- Berkeley College (US)
- Lawrence Berkeley National Laboratory (US)
- University of California, San Francisco (US)
- Baylor College of Medicine (US)
- University of Chicago (US)
- Allen Institute (US)
- Nano and Advanced Materials Institute (CN)
- Rice University (US)
- University of San Francisco (US)
- University of Hong Kong (HK)
- University of California, Berkeley (US)
Publication Details
- Journal
- Nature Methods
- Published
- 2025-12-09
- DOI
- https://doi.org/10.1038/s41592-025-02925-7
- Citations
- 2
- Primary Topic
- Advanced Fluorescence Microscopy Techniques
- Type
- article
- Field-Weighted Citation Impact
- 3.27