A high‐throughput bone marrow <scp>3D</scp> co‐culture system to study resistance to <scp>BCR</scp> signalling targeted agents in B‐ <scp>NHL</scp>
Bone marrow (BM) involvement in B-cell non-Hodgkin lymphoma (B-NHL) is associated with poor prognosis, as the BM microenvironment provides a protective niche that promotes therapeutic resistance. We developed a simplified, automated and high-throughput 3D BM co-culture model that faithfully reproduces key tumour-stroma interactions. In our system, BM stromal cells (BMSCs) decreased lymphoma cell sensitivity to Phosphatidylinositol 3-kinase (PI3K) and BTK inhibitors. Moreover, we show that our 3D platform enables the mechanistic studies of microenvironment-mediated drug resistance and has the potential to be developed into a tool for personalized therapeutic strategies for B-NHL.
Authors
- Matteo Moretti (ORCID: https://orcid.org/0000-0002-7301-1208)
- Christian Candrian (ORCID: https://orcid.org/0000-0002-3543-981X)
- Alberto J. Arribas (ORCID: https://orcid.org/0000-0003-3123-6203)
- Afua A. Mensah (ORCID: https://orcid.org/0000-0001-8113-3689)
- Filippo Spriano (ORCID: https://orcid.org/0000-0002-8615-1568)
- Eleonora Cannas (ORCID: https://orcid.org/0009-0000-9551-7122)
- Chiara Arrigoni (ORCID: https://orcid.org/0000-0001-6808-1642)
- Maria Vittoria Colombo (ORCID: https://orcid.org/0000-0002-6018-9920)
- Francesco Bertoni (ORCID: https://orcid.org/0000-0001-5637-8983)
- Dalila Petta (ORCID: https://orcid.org/0000-0002-1412-0084)
- Federico Simonetta (ORCID: https://orcid.org/0000-0002-0399-689X)
- Luciano Cascione
- Alex Zadro (ORCID: https://orcid.org/0009-0003-1322-891X)
Institutions
- University of Geneva (CH)
- SIB Swiss Institute of Bioinformatics (CH)
- Swiss Institute for Regenerative Medicine (CH)
- Ente Ospedaliero Cantonale (CH)
- Institute of Oncology Research (CH)
- University Hospital of Geneva (CH)
- IRCCS Ospedale Galeazzi - Sant'Ambrogio (IT)
- Istituto Oncologico della Svizzera Italiana
- Università della Svizzera italiana (CH)
- Geneva College (US)
Publication Details
- Journal
- British Journal of Haematology
- Published
- 2025-12-08
- DOI
- https://doi.org/10.1111/bjh.70273
- Primary Topic
- Lymphoma Diagnosis and Treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00