A telomere-to-telomere genome assembly for Meyerozyma guilliermondii ATCC 6260: closing gaps and resolving a translocation in the reference sequence

<i>Meyerozyma guilliermondii,</i> a haploid yeast (phylum Ascomycota), is useful in bioprocessing and bioremediation, and is an infrequent pathogen of humans. The availability of highly accurate, long-read DNA sequencing methods provided the opportunity to improve the <i>M. guilliermondii</i> genome assembly. Using Pacific Biosciences (PacBio) HiFi technology, we generated a chromosome-level, telomere-to-telomere assembly for the <i>M. guilliermondii</i> type strain ATCC 6260. This assembly closed gaps in the current reference sequence and resolved a translocation artefact that affected the size and structure of chromosomes 4 and 5. The improved genome sequence is available publicly and will facilitate future studies of this species.