Jiella marina sp. nov., isolated from the seawater in a hydrothermal field of the Okinawa Trough

A novel marine bacterium, strain LLJ827^T, was isolated from deep-sea waters from the Yonaguni Knoll IV hydrothermal vent in the Okinawa Trough. This Gram-stain-negative, strictly aerobic, rod-shaped organism exhibits a growth temperature range of 20-45 °C, with the optimal growth at 28 °C. The strain thrives in pH conditions ranging from 5.0 to 8.0 (optimal at pH 6.0) and can tolerate NaCl concentrations of 3-10%. The predominant respiratory of strain LLJ827^T was ubiquinone-10. The major fatty acids identified were summed feature 8 (comprising C_18:1 <i>ω</i>7<i>c</i> and/or C_18:1 <i>ω</i>6<i>c</i>) and C_18:1 2-OH. The polar lipid profile included phosphatidylcholine (PC), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylmonomethylethanolamine (PME), along with several unidentified phospholipids (PL), aminolipids (AL), glycolipids (GL) and lipids (L). The 16S rRNA gene sequence of strain LLJ827^T showed the closest similarity to <i>Jiella avicenniae</i> CBK1P-4^T (98.06%), followed by other <i>Jiella</i> species. Phylogenetic analysis based on 16S rRNA gene sequences also showed that strain LLJ827^T was clustered within the genus <i>Jiella</i>. The average nucleotide identity and DNA-DNA hybridization values of genomes of strain LLJ827^T and <i>J. avicenniae</i> CBK1P-4^T are 77.21 and 22.3%, respectively. The genomic G+C content of strain LLJ827^T is 64.0 mol%. Strain LLJ827^T has numbers of genes associated with carbohydrate metabolism, thiosulphate oxidation and [NiFe]-hydrogenases. Based on comprehensive polyphasic analysis, strain LLJ827^T is proposed as a novel species within the <i>Jiella</i> genus, named <i>Jiella marina</i> sp. nov., with the type strain designated as LLJ827^T (=MCCC 1K04957^T=KCTC 82884^T).