Efficient Decolorization and Preparation of Sparassis crispa Polysaccharides Using Amino-Modified Silica Gel and Evaluation of Their Biological Activites

This study synthesized an efficient amino-modified silica gel decolorizer by aminating silica hydroxyls, characterized via SEM, FT-IR, N₂ adsorption and XPS. It investigated its decolorization of <i>Sparassis crispa</i> crude polysaccharides using decolorization rate and polysaccharide retention as indices, revealing pigment adsorption mechanisms. Polysaccharide activity preservation by physical adsorption was evaluated via antioxidant (DPPH, ABTS, •OH scavenging) and zebrafish caudal fin repair assays. The results showed 79.85% decolorization and 86.54% polysaccharide retention within 5 min, with over 75% decolorization after five cycles of reuse. Results suggest that pigment adsorption may involve interactions between amino groups and chromophoric compounds. The decolorized polysaccharides showed better antioxidant and zebrafish caudal fin repair activities than those treated with traditional H₂O₂. These findings support the development of efficient, low-damage decolorization strategies for edible and medicinal polysaccharides.