Protocol to investigate fibroblastic reticular cell-immune cell interaction in human lung cancer
Fibroblastic reticular cells (FRCs) play critical roles in the generation of anti-tumor immune responses. Here, we present a protocol for characterizing FRCs in human lung cancer. This step-by-step protocol involves enrichment of rare FRCs from tumor tissue for single-cell RNA sequencing and a detailed computational framework for the analysis of FRC-immune cell crosstalk. The topography and physical interactions of FRCs and T cells in specific niche environments are validated using optimized immunohistochemical and high-resolution microscopy approaches. For complete details on the use and execution of this protocol, please refer to Onder et al.<sup>1</sup>.
Authors
- Almut Lütge (ORCID: https://orcid.org/0000-0003-1104-727X)
- Mechthild Lütge (ORCID: https://orcid.org/0000-0001-9011-5319)
- Mark D. Robinson (ORCID: https://orcid.org/0000-0002-3048-5518)
- Burkhard Ludewig (ORCID: https://orcid.org/0000-0002-7685-573X)
- Lucas Onder (ORCID: https://orcid.org/0000-0003-1471-412X)
- Angelina De Martin (ORCID: https://orcid.org/0000-0002-9056-8075)
- Chrysa Papadopoulou (ORCID: https://orcid.org/0000-0002-3909-7378)
- Hung‐Wei Cheng (ORCID: https://orcid.org/0000-0003-1740-0337)
Institutions
- SIB Swiss Institute of Bioinformatics (CH)
- University of Zurich (CH)
- University of St.Gallen (CH)
- University Hospital of Zurich (CH)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1016/j.xpro.2025.104256
- Primary Topic
- Single-cell and spatial transcriptomics
- Type
- article
- Field-Weighted Citation Impact
- 0.00