Isolation and characterization of myo inositol from an agricultural waste—rice bran

This study focusses on isolation of myo inositol crystals from rice bran (<i>Oryza sativa. indica,</i> Indian variety<i>)</i> an agricultural by-product, using an acid hydrolysis process optimized through response surface methodology (RSM). The obtained crystals were characterized using X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and high-performance liquid chromatography (HPLC) and nuclear magnetic resonance (NMR). XRD analysis revealed a well-defined crystalline structure with a dominant diffraction peak at 31.74°, indicating high crystallinity. FTIR spectra verified the presence of characteristic hydroxyl and C-O functional groups of myo inositol. HPLC analysis demonstrated approximately 80% purity. The ¹H-NMR spectroscopy was employed to analyze the structural identity of the isolated myo inositol crystals. The spectrum exhibited the characteristic four signal groups with relative intensities of 1:2:2:1, consistent with the molecular symmetry of myo inositol (C₆H₁₂O₆). Proton assignments based on chemical shifts and coupling patterns (H2-H4-H6-H1-H3-H5) corresponded closely with previously reported spectra, confirming successful isolation of structurally pure myo inositol. This study presents a simple and efficient method for recovering myo inositol from <i>Oryza sativa. indica</i> bran, offering a sustainable approach to valorize agro-industrial waste for potential nutraceutical and pharmaceutical applications.