Protocol for Seahorse 3D Mito Stress assay in patient-derived pediatric brain tumor single neurospheres
Optimization of Seahorse assay protocol for single patient-derived brain tumor spheroidsStep-by-step instructions for successfully re-plating and transferring single brain tumor spheroidsImportance of microplate coating for the successful execution of Seahorse assaysImaging of the microplate before and after the assay allows for normalization of metabolic parameters based on neurosphere size.
Authors
- Jessica W. Tsai (ORCID: https://orcid.org/0000-0003-0540-4330)
- Stefania Tocci
Institutions
- University of Southern California (US)
- Children's Hospital of Los Angeles (US)
- USC Norris Comprehensive Cancer Center (US)
Publication Details
- Journal
- bioRxiv (Cold Spring Harbor Laboratory)
- Published
- 2025-12-05
- DOI
- https://doi.org/10.64898/2025.12.03.691986
- Primary Topic
- Aquatic life and conservation
- Type
- article
- Field-Weighted Citation Impact
- 0.00