Evaluating Effect of Aqueous Extract of Seeds of Raphanus Sativus on Histological Changes in Kidney Resulting from Complications of Diabetes in Rats

Study aimed to know effect of aqueous extract of radish seeds and its role in reducing and maintaining levels of glucose, insulin hormone, and effect on kidney functions represented by levels of creatinine and urea in blood, as well as its role in alleviating tissue changes in kidneys. Twenty-four rats were used and divided into three equal groups: first (non-diabetic), second group, control group, in which experimental diabetes was induced with streptozotocin at a dose of 50 mg/kg, and third group, diabetic, treated with aqueous extract at a dose of 300 mg/kg for two periods (three and six weeks). After that, blood samples were collected to measure levels of glucose, insulin hormone, urea and creatinine. Tissue changes in kidneys were also examined. Results showed a significant difference in glucose levels for diabetic control group after injection with STZ and a significant decrease in aqueous radish group after six weeks compared to period after STZ injection. Results also indicate a significant decrease in concentration of Insulin hormone after induction compared to healthy control group. After six weeks of treatment with extract, an increase in level of insulin hormone was observed compared to period after injection. Results also indicate an increase in level of urea and creatine in blood serum after inducing diabetes. After a period of treatment with this extract, a decrease in level of urea and creatine was observed compared to period after injection.