Metabolomic-based promising technique for fruit-based natural products analysis

Fruit-based natural products have significant health and commercial benefits. Liquid chromatography-high-resolution mass spectrometry (LC-HRMS) is a powerful technique to profile complex chemical compositions. This review focuses on the use of LC-HRMS for profiling fruit-derived bioactive chemicals and demonstrates the advantages of LC for separation and HRMS for precise mass detection. LC-HRMS enables the detection of a wide range of bioactive compounds, while providing excellent precision and resolution, allowing distinction between isomeric and isobaric compounds and advancing the understanding of fruit metabolomes and their roles in food and pharmaceutical sciences. Although challenges such as complex sample preparation and limited databases persist, LC-HRMS continues to be the leading method for discovering novel bioactive compounds. Continuous improvements in analytical procedures and data resources are required to fully realize the potential of LC-HRMS. Overall, the integration of LC and HRMS has altered fruit-based analyses of natural products, providing their chemical profiles and encouraging innovations in health-related and industrial applications.