Efficient refolding, purification, and characterization of barley oxalate oxidase in Escherichia coli
Our study highlights the feasibility of using E. coli for the expression and purification of OxOx, providing a promising approach for future applications in the field of healthcare. Our study has also laid the foundation for further developing an oxalate test strip for urinary oxalate assays based on OxOx.
Authors
- Benjamin K. Canales (ORCID: https://orcid.org/0000-0002-9571-8394)
- William Donelan (ORCID: https://orcid.org/0000-0001-7742-8089)
- ShiWu Li
- Paul R. Dominguez‐Gutierrez (ORCID: https://orcid.org/0000-0002-5414-6895)
- Dongqi Tang (ORCID: https://orcid.org/0000-0002-1429-9467)
- Lijun Yang (ORCID: https://orcid.org/0000-0002-9525-0090)
Institutions
- University of Florida (US)
- Second Hospital of Shandong University (CN)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-12-06
- DOI
- https://doi.org/10.1186/s12934-025-02775-0
- Primary Topic
- Kidney Stones and Urolithiasis Treatments
- Type
- article
- Field-Weighted Citation Impact
- 0.00