Draft genomes of two Streptococcus agalactiae from bovine mastitis in the North-West Province, South Africa

Genomic DNA of S. agalactiae strains was extracted and sequenced using the Oxford Nanopore MiniON platform. De novo assembly was carried out using Flye v. v2.9, resulting in an average draft genome size of 2.25 Mb for both strains, assembled into 4 contigs for strain 40LHNT and 91 contigs for strain 10RH2NT with an average GC content of 35.5% for both strains. Genome quality assessment revealed an average completeness of 92.64% and a contamination level of 1.6%. For both strains, the closest reference type strain was S. agalactiae NCTC8181 (accession GCA_900458965.1), showing an average nucleotide identity of 97%, thereby confirming species-level identity and close genetic relatedness. Annotation using the NCBI Prokaryotic Genome Annotation Pipeline identified 2,067 and 1977 protein-coding genes for strains 40LHNT and 10RH2NT, respectively..