Rescue of extreme hepatectomy mice by primary hepatocyte-derived 3D bio-printed organ transplantation
Three-dimensional (3D) bioprinting is an emerging strategy for constructing tissues and organs<i>in vitro</i>. Here, we achieved long-term expansion of primary mouse hepatocytes using a defined medium and constructed liver tissue using 3D bioprinting. The 3D-printed liver tissue demonstrated several essential liver functions and was able to prolong the survival of mice with acute liver failure due to extreme hepatectomy after<i>in vivo</i>transplantation, and the transplanted artificial liver tissue showed distinct functional partitioning. Overall, our results develop a method for long-term<i>in vitro</i>culture of primary hepatocytes and demonstrate the potential of 3D bio-printed liver tissue for clinical translational applications.
Authors
- Changcan Li
- Lejia Sun (ORCID: https://orcid.org/0000-0002-7161-1103)
- Yi Lu (ORCID: https://orcid.org/0009-0004-4638-9178)
- Mingchang Pang
- Z. T. He (ORCID: https://orcid.org/0000-0001-9167-6986)
- Xindi Ke (ORCID: https://orcid.org/0000-0001-6970-782X)
- Hang Sun (ORCID: https://orcid.org/0000-0003-1565-0308)
- Shunda Du (ORCID: https://orcid.org/0000-0002-9357-3259)
- Pengyu Huang (ORCID: https://orcid.org/0000-0001-8394-7280)
- Xinting Sang (ORCID: https://orcid.org/0000-0003-1952-0527)
- Huayu Yang (ORCID: https://orcid.org/0000-0001-9791-3559)
- Yinhan Wang (ORCID: https://orcid.org/0000-0003-2604-9040)
- Haitao Zhao (ORCID: https://orcid.org/0000-0002-3444-8044)
- Yilei Mao (ORCID: https://orcid.org/0000-0003-0449-4223)
- Y. Ye
- Haifeng Xu (ORCID: https://orcid.org/0000-0003-2857-2628)
- Bao Jin (ORCID: https://orcid.org/0000-0002-7488-8204)
- Zhiyuan Fang (ORCID: https://orcid.org/0000-0001-5622-8918)
- Zhao-Xiong Xie (ORCID: https://orcid.org/0000-0001-5063-9070)
- Yaodan Chi
- Zimin Jiang (ORCID: https://orcid.org/0000-0002-9548-4348)
Institutions
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Beijing Chao-Yang Hospital, Capital Medical University (CN)
- Peking Union Medical College Hospital (CN)
- University of Oxford (GB)
- Science Oxford (GB)
- Beijing Friendship Hospital (CN)
- Tianjin Medical University (CN)
- Nanjing Medical University (CN)
Publication Details
- Journal
- Biofabrication
- Published
- 2025-12-05
- DOI
- https://doi.org/10.1088/1758-5090/ae288d
- Primary Topic
- 3D Printing in Biomedical Research
- Type
- article
- Field-Weighted Citation Impact
- 0.00