Association between hypertensive patients of Kazakh nationality in Xinjiang and T lymphocytes microRNAs

To explore the relationship between microRNAs (miRNAs) in T lymphocytes and primary hypertension in Kazakh people. Five Kazakh patients with primary hypertension who were initially diagnosed and untreated, as well as 5 healthy individuals who underwent physical examinations during the same period, were selected. Plasma samples of the subjects were collected, and T lymphocytes were isolated. High throughput second-generation sequencing technology was used to detect changes in miRNA expression in the two groups of subjects, and differentially expressed miRNAs were screened for further analysis of lymphocytes IL-6, IL-17, and interferon-gamma (IFN-γ), tumor necrosis factor-alpha (TNF-α) mRNA expression level detection; Serum IL-6, IL-17, IFN-γ, TNF-α detection of cytokine levels; Correlation analysis and clinical value prediction between differential expression of miRNAs and mean arterial pressure in patients. A total of 1754 miRNAs were detected in this study. Compared with the control group, 33 miRNAs were upregulated in the hypertension group (P < .05), and 60 miRNAs were downregulated (P < .05). Verify 6 miRNAs in the sample population: miR-15b-5p, miR-374-5p, miR-138-5p, miR-320a-3p, miR-423-5p, and miR-92a-3p. The quantitative real-time polymerase chain reaction results showed that among the 6 differentially expressed miRNAs, miR-320a-3p, miR-423-5p, and miR-92a-3p were downregulated in the hypertensive group (P < .05). Lymphocyte IFN in hypertensive group-γ, The expression level of IL-17 mRNA increases, and IL-6 and IFN in plasma-γ, The level of IL-17 has increased. The area under the miRNAs curve for differential expression of lymphocytes in Kazakh hypertensive patients, where miR-423-5p, area under the curve (AUC): 0.696 (95% confidence interval (CI): 0.533-0.859); MiR-320a-3p, AUC: 0.764 (95% CI: 0.609-0.919). The expression of miR-92a-3p, miR-423-5p, and miR-320a-3p in T lymphocytes of Kazakh hypertensive patients is downregulated, with good diagnostic reference value (P < .05).