Highly Efficient Multiplex Precise Genome Editing in Porcine Cells Using epiCBE4max System
1. EpiCBE4max, a high-efficiency, one-step multiplex base editing system, was developed by integrating episomal elements with the CBE4max platform. 2. The epiCBE4max system achieved a simultaneous homozygous editing efficiency of 44.44% across five target genes in porcine fetal fibroblasts with undetectable off-target effects.
Authors
- Shengsong Xie (ORCID: https://orcid.org/0000-0002-8301-877X)
- Changchun Li (ORCID: https://orcid.org/0000-0002-8030-8632)
- Xinyun Li (ORCID: https://orcid.org/0000-0002-7943-1713)
- Xiaosong Han (ORCID: https://orcid.org/0000-0001-7353-5186)
- Jinxue Ruan (ORCID: https://orcid.org/0000-0002-0058-6924)
- Xianghua Xu (ORCID: https://orcid.org/0000-0001-5244-6707)
- Jing Lü (ORCID: https://orcid.org/0000-0002-5834-7942)
- Xuewen Xu (ORCID: https://orcid.org/0000-0001-6925-3346)
- Bo Li (ORCID: https://orcid.org/0000-0002-9471-9168)
Institutions
- Huazhong Agricultural University (CN)
- Ministry of Agriculture and Rural Affairs (CN)
- New Hope Liuhe (China) (CN)
Publication Details
- Journal
- Journal of Integrative Agriculture
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1016/j.jia.2025.12.011
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.00