Echinocandin tolerance and persistence <i>in vitro</i> are regulated by calcineurin signaling in <i>Candida glabrata</i>

Treatment of fungal infections is often unsuccessful. Potential causes of antifungal failure include tolerance and persistence, which are poorly understood processes used by fungal pathogens to survive antifungal treatment. This study utilizes detailed experimental protocols and genome-wide screens to discover how <i>Candida glabrata</i> induces tolerance and persistence to a major class of antifungals. The findings suggest that a clinical immunosuppressant may be repurposed to combat tolerance and persistence in this pathogenic yeast, as well as <i>Candida albicans</i> and perhaps other species.