An evaluation of screening methods for the detection of extended-spectrum beta-lactamase-producing <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> in environmental samples from healthcare settings
Pre-enrichment for 18 h in BPW, followed by plating onto cefotaxime-supplemented MLGA, was the most effective screening method in our context, having a high detection efficacy, whilst maintaining a scalable and accessible cost.
Authors
- Maria Moore (ORCID: https://orcid.org/0000-0001-6456-927X)
- Nicholas Feasey (ORCID: https://orcid.org/0000-0003-4041-1405)
- Joseph M. Lewis (ORCID: https://orcid.org/0000-0002-3837-5188)
- Claudia McKeown (ORCID: https://orcid.org/0009-0002-2823-5461)
- Sarah Gallichan (ORCID: https://orcid.org/0000-0001-8938-1628)
- Stephen R. Forrest (ORCID: https://orcid.org/0000-0003-0131-1903)
- Fabrice E. Graf (ORCID: https://orcid.org/0000-0003-1132-5348)
- Esther Picton-Barlow (ORCID: https://orcid.org/0009-0002-1189-4751)
Institutions
- Andrews University (US)
- University of Liverpool (GB)
- University of St Andrews (GB)
- Liverpool School of Tropical Medicine (GB)
- Royal Liverpool University Hospital (GB)
Publication Details
- Journal
- Journal of Applied Microbiology
- Published
- 2025-11-28
- DOI
- https://doi.org/10.1093/jambio/lxaf295
- Primary Topic
- Antibiotic Resistance in Bacteria
- Type
- article
- Field-Weighted Citation Impact
- 0.00