Evaluation of a lateral flow immunochromatographic assay for detecting cryptococcal antigens in bronchoalveolar lavage fluid from HIV-negative patients with pulmonary cryptococcosis

This study demonstrates the significant diagnostic value of the cryptococcal antigen lateral flow immunochromatographic assay (CrAg-LFA) for pulmonary cryptococcosis (PC) in human immunodeficiency virus (HIV)-negative patients. We found that CrAg-LFA performed on bronchoalveolar lavage fluid (BALF) was highly effective, with a sensitivity of 88.1% and a specificity of 99.6%. Importantly, combining BALF and serum tests increased sensitivity and negative predictive value to 100%, providing a more reliable diagnostic approach. These findings highlight the utility of BALF CrAg-LFA in facilitating early and accurate PC diagnosis, which can significantly improve patient outcomes in non-HIV populations. This research offers critical insights into optimizing diagnostic strategies for this challenging condition.

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Publication Details

Journal
Microbiology Spectrum
Published
2025-12-05
DOI
https://doi.org/10.1128/spectrum.01083-25
Citations
1
Primary Topic
Fungal Infections and Studies
Type
article
Field-Weighted Citation Impact
1.35
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article

Evaluation of a lateral flow immunochromatographic assay for detecting cryptococcal antigens in bronchoalveolar lavage fluid from HIV-negative patients with pulmonary cryptococcosis

Huiying Zhao, Jie Yu, Hongbin Chen, Qi Wang et al.
1 citations
Microbiology Spectrum
Fungal Infections and Studies
1.35
article

Evaluation of a lateral flow immunochromatographic assay for detecting cryptococcal antigens in bronchoalveolar lavage fluid from HIV-negative patients with pulmonary cryptococcosis

Huiying Zhao, Jie Yu, Hongbin Chen, Qi Wang, Ke Wang, Feifei Zhang, Zhanwei Wang, Zhixiang Li, Jiangfan Ran, Xin Chen, Chun Di, Hui Wang
article en
1 citations

Abstract

This study demonstrates the significant diagnostic value of the cryptococcal antigen lateral flow immunochromatographic assay (CrAg-LFA) for pulmonary cryptococcosis (PC) in human immunodeficiency virus (HIV)-negative patients. We found that CrAg-LFA performed on bronchoalveolar lavage fluid (BALF) was highly effective, with a sensitivity of 88.1% and a specificity of 99.6%. Importantly, combining BALF and serum tests increased sensitivity and negative predictive value to 100%, providing a more reliable diagnostic approach. These findings highlight the utility of BALF CrAg-LFA in facilitating early and accurate PC diagnosis, which can significantly improve patient outcomes in non-HIV populations. This research offers critical insights into optimizing diagnostic strategies for this challenging condition.

Microbiology SpectrumVol. 14(1)
King University (US), Nanchang University (CN), Peking University (CN), Inner Mongolia University (CN), The Fourth People's Hospital (CN), Foshan Hospital of TCM (CN), Second Affiliated Hospital of Nanchang University (CN), Chinese University of Hong Kong, Shenzhen (CN), Peking University People's Hospital (CN), Inner Mongolia People's Hospital (CN), University of Hong Kong - Shenzhen Hospital (CN), Fourth Hospital of Inner Mongolia (CN), Shenzhen Second People's Hospital (CN)
Openalex Percentile: Top 13%
Fungal Infections and Studies
1.35
DOIOpen Access PDFOpenAlex
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