Earlier de-isolation of SARS-CoV-2-infected ICU patients using a novel viability PCR: a prospective cohort study
This study demonstrated that, within patients, on average, viability PCR became negative 2.4 days earlier than conventional PCR, indicating that viability PCR could potentially assist in de-isolating patients 2.4 days earlier. Future use of this assay could thus aid in improving routine COVID-19 diagnostics and prognostics related to the timing of de-isolation. Additionally, future development of a similar approach for other respiratory viruses could be of interest.
Authors
- Walther van Mook (ORCID: https://orcid.org/0000-0003-2398-8878)
- Petra Wolffs (ORCID: https://orcid.org/0000-0002-5326-3985)
- Ruben Deneer (ORCID: https://orcid.org/0000-0002-6011-7783)
- Tom Schoenmakers (ORCID: https://orcid.org/0000-0002-1576-7832)
- Una Vojinović (ORCID: https://orcid.org/0009-0008-6304-1084)
- Wilhelmine P.H.G. Verboeket‐van de Venne (ORCID: https://orcid.org/0000-0003-4980-0116)
- Bas C. T. van Bussel (ORCID: https://orcid.org/0000-0003-1621-7848)
- Frank van Rosmalen (ORCID: https://orcid.org/0000-0002-9522-3711)
- Stefan H. M. Gorissen (ORCID: https://orcid.org/0000-0003-3737-9053)
- Mathie P.G. Leers (ORCID: https://orcid.org/0000-0001-5186-5600)
- Paul H. M. Savelkoul (ORCID: https://orcid.org/0000-0002-3527-6950)
- Inge H. M. van Loo (ORCID: https://orcid.org/0000-0002-5960-4357)
Institutions
- Radboud University Nijmegen (NL)
- Maastricht University Medical Centre (NL)
- Catharina Ziekenhuis (NL)
- Cardiovascular Institute Hospital (JP)
- Maastricht University (NL)
- Institute for Postgraduate Medical Education (CZ)
- Zuyderland Medisch Centrum (NL)
- Open University of the Netherlands (NL)
- Eindhoven University of Technology (NL)
Publication Details
- Journal
- Microbiology Spectrum
- Published
- 2025-12-05
- DOI
- https://doi.org/10.1128/spectrum.01140-25
- Primary Topic
- SARS-CoV-2 detection and testing
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- ZonMw