Colorectal cancer organoids drive hypoxia, TGF-β, and patient-specific diversification of NK cell activation programs
The NK cell/PDO co-culture platform allows the identification of both common and patient-specific impacts of the tumor microenvironment on NK cell function and can aid the development of patient-tailored immunotherapies. The majority of CRC (CMS2/CMS3) PDOs from our cohort were susceptible to NK cell-mediated killing and induced NK cell activation, highlighting the potential of NK cells for CRC immunotherapies.
Authors
- Ana Stojanovic (ORCID: https://orcid.org/0000-0001-9503-2256)
- Johannes Betge (ORCID: https://orcid.org/0000-0001-9549-1866)
- Irene Garces (ORCID: https://orcid.org/0000-0003-0834-082X)
- Adelheid Cerwenka (ORCID: https://orcid.org/0000-0001-6977-3536)
- Elke Burgermeister (ORCID: https://orcid.org/0000-0002-4969-5697)
- Tomáš Hofman (ORCID: https://orcid.org/0000-0003-2574-9686)
- Laura Helming (ORCID: https://orcid.org/0009-0005-6268-2101)
- Kim E. Boonekamp (ORCID: https://orcid.org/0000-0002-9968-5382)
- Michael Boutros (ORCID: https://orcid.org/0000-0002-9458-817X)
- Carsten Sticht (ORCID: https://orcid.org/0000-0003-3596-6094)
- Indra A. Shaltiël (ORCID: https://orcid.org/0000-0002-5673-6741)
- Andreas von Kries (ORCID: https://orcid.org/0000-0002-7425-2329)
- Bianca M Balzasch (ORCID: https://orcid.org/0009-0006-0038-4413)
- Matthias Ebert (ORCID: https://orcid.org/0000-0001-5713-5465)
- Annika Sams (ORCID: https://orcid.org/0009-0004-7953-6146)
- Alessia Triassi (ORCID: https://orcid.org/0009-0008-5020-7698)
Institutions
- European Molecular Biology Organization (DE)
- University of Mannheim (DE)
- German Cancer Research Center (DE)
- Heidelberg University (DE)
- University Hospital Heidelberg (DE)
- Ono Pharmaceutical (United States) (US)
- Research & Development Institute (US)
- DKFZ-ZMBH Alliance (DE)
- University Medical Centre Mannheim (DE)
- Mannheim Centre for European Social Research (DE)
Publication Details
- Journal
- Journal for ImmunoTherapy of Cancer
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1136/jitc-2025-012988
- Primary Topic
- Immune Cell Function and Interaction
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Merck KGaA
- European Commission
- Deutsche Forschungsgemeinschaft
- Deutsche Krebshilfe
- Horizon 2020 Framework Programme