Aloe Vera alleviates glyphosate-based herbicide hepatotoxicity in rats via anti-inflammatory and antioxidant actions

Rats exposed to GBH showed significant oxidative stress, evidenced by elevated malondialdehyde (MDA) and decrease the reduced glutathione (GSH) levels, alongside increased the inflammatory markers (TNF-<i>α</i> and IL-1β). Liver function enzymes (ALT, AST, LDH) were markedly elevated, and comet assay results revealed substantial DNA fragmentation in hepatocytes. Histopathological findings and immunohistochemical overexpression of caspase-3 confirmed the liver pathological lesions and apoptosis, respectively. Co-administration of <i>Aloe Vera</i> with GBH significantly alleviated the biochemical, histopathological, and apoptotic alterations, but it did not fully reverse the DNA damage. These findings suggest that <i>Aloe Vera</i> may offer partial hepatoprotection against GBH toxicity through its antioxidant and anti-inflammatory properties.