GLANCE-aided snapshotting for sustainable integration of synchronous spectrofluorimetry and micellar boosting for nanoscale assay of tolterodine binary mixtures in crucial matrices

Tolterodine tartrate (TLD) has recently demonstrated remarkable synergism when combined with either of two α₁-adrenergic antagonists, doxazosin mesilate (DXZ) or terazosin hydrochloride dihydrate (TRZ), for treating urinary tract issues. Herein, a sustainability-oriented approach is adopted for integrating constant wavelength synchronous fluorescence with micellar augmentation for ultra-trace tracking of TLD in combination with DXZ (mixture I) or TRZ (mixture II). The designed protocol relies chiefly on exploiting sodium dodecyl sulfate as a biodegradable fluorescence enhancer and water as an eco-friendly diluting solvent, maintaining a fixed wavelength difference (Δλ = 20 nm) to record the drugs' signals. Under optimal experimental conditions, favorable linearity at the nano level was achieved over concentration ranges of 20.0-200.0, 5.0-50.0, and 5.0-50.0 ng mL⁻¹, with remarkable LOD (1.25, 0.90, and 0.73 ng mL⁻¹) and LOQ (3.78, 2.73, and 2.20 ng mL⁻¹) levels for TLD, DXZ, and TRZ, respectively. The methodology effectively evaluated the cited drugs per se, in their laboratory-prepared mixtures, and in dosage form, without impediments from one another or the tablet excipients. The outstanding ultra-sensitivity of the developed strategy permitted concurrent minute analysis of the targeted drugs in human plasma, with acceptable standard deviation (SD) values ≤ 2.40. Additionally, exceptional analytical performance has been demonstrated for the nominated medications in two environmental samples with SD values ranging from 0.59 to 1.57. The proposed work prioritizes sustainability principles by excluding harsh chemicals and convoluted procedures, constituting a cornerstone of the design. These principles were checked using: MoGAPI, AGREE, CACI, RGBfast, RAPI, and BAGI. Interestingly, the cutting-edge GLANCE tool was employed to display a quick snapshot of the framework aspects in a well-organized template, without digging into overwhelming datasets. The outcomes demonstrated the excellence of the designed methodology over reported approaches, offering proficient sensitivity, sustainability, facility, and practicability across diverse crucial matrices. These findings provide compelling evidence that the proposed work serves as an efficient, eco-friendly quantification tool for use in quality control, biological, and environmental sectors, fostering a more eco-conscious and sustainable scientific community.