Interspecies rice <i>versus Arabidopsis thaliana</i> protein–protein interactome profiling by touch‐down overlapping PCR coupled with HiFi long‐read sequencing
Touch-down overlapping PCR coupled with HiFi long-read sequencing, a high-throughput method for large-scale profiling of protein-protein interactions based on stitch-PCR identified 7,726 high-confidence interactions between rice and Arabidopsis proteins by integrating a library-vs-library yeast two-hybrid strategy with optimized PCR and long-read sequencing.
Authors
- Dawei Xue (ORCID: https://orcid.org/0000-0001-9904-7615)
- Dandan Xia (ORCID: https://orcid.org/0009-0008-5576-1612)
- Jian Zhang (ORCID: https://orcid.org/0000-0003-2804-0162)
- Delong Fan (ORCID: https://orcid.org/0009-0000-9319-1958)
- X.M. Liu
- Xiaohong Tong (ORCID: https://orcid.org/0000-0002-6580-5358)
- Yuxuan Hou (ORCID: https://orcid.org/0000-0002-0830-4717)
- Jiezheng Ying (ORCID: https://orcid.org/0000-0001-8457-8405)
- Yu Cheng (ORCID: https://orcid.org/0009-0002-3303-9342)
- Jianwei Zhang (ORCID: https://orcid.org/0000-0001-8030-5346)
- Yifeng Wang (ORCID: https://orcid.org/0000-0002-5187-3755)
- Yi‐Ting Chen (ORCID: https://orcid.org/0009-0006-4429-927X)
- Zhiyong Li (ORCID: https://orcid.org/0000-0002-1309-7732)
- Jie Huang
- J. Ruan (ORCID: https://orcid.org/0009-0009-1486-2350)
Institutions
- Hangzhou Normal University (CN)
- Huazhong Agricultural University (CN)
- China National Rice Research Institute (CN)
Publication Details
- Journal
- Journal of Integrative Plant Biology
- Published
- 2025-12-04
- DOI
- https://doi.org/10.1111/jipb.70107
- Primary Topic
- Bioinformatics and Genomic Networks
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Chinese Academy of Agricultural Sciences
- Huazhong Agricultural University
- National Science and Technology Major Project
- Agricultural Science and Technology Innovation Program
- Natural Science Foundation of Zhejiang Province