Crystal and Cryo-EM structure of PPL, a novel hexameric R-type lectin from the poisonous mushroom <i>Pleurocybella porrigens</i>
Pleurocybella porrigens is a mushroom that grows widely around the temperate northern hemisphere, and was once considered edible, especially in Japan. Following a number of deaths in 2004, investigations revealed the presence of various toxins, including a lectin (PPL) that apparently survives cooking and enters the bloodstream via the stomach. We have cloned PPL and solved its structure by X-ray crystallography and cryo-EM. We report the sugar binding properties of this β-trefoil lectin, which has a novel hexameric structure.
Authors
- Arnout R. D. Voet (ORCID: https://orcid.org/0000-0002-3329-2703)
- Jeremy R. H. Tame (ORCID: https://orcid.org/0000-0002-9341-7280)
- Kenichi Kamata (ORCID: https://orcid.org/0000-0003-4344-8473)
- Sam‐Yong Park
- Naito Ishimoto (ORCID: https://orcid.org/0000-0001-8976-8582)
- K. Mizutani (ORCID: https://orcid.org/0000-0002-7337-2134)
- Masao Yamada (ORCID: https://orcid.org/0009-0005-2236-4766)
- Katsuya Takahashi (ORCID: https://orcid.org/0000-0003-2028-244X)
- Yasuhiro Ozeki (ORCID: https://orcid.org/0000-0002-2782-6158)
- Laurens Vandebroek (ORCID: https://orcid.org/0000-0001-6907-425X)
- Yuki Fujii (ORCID: https://orcid.org/0009-0005-6379-9600)
- Hideaki Fujita (ORCID: https://orcid.org/0000-0002-4105-4430)
- Daisuke Adachi
- Hironori KAWABATA
- Reiji Kubota
Institutions
- Nihon University (JP)
- Chiba University (JP)
- Nagasaki International University (JP)
- Yokohama City University Medical Center (JP)
- Yokohama City University (JP)
- KU Leuven (BE)
Publication Details
- Journal
- Glycobiology
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1093/glycob/cwaf082
- Primary Topic
- Silymarin and Mushroom Poisoning
- Type
- article
- Field-Weighted Citation Impact
- 0.00