Directed evolution–driven reprogramming of PD-L1 for compact and tunable checkpoint modulation
This study demonstrates the successful application of directed evolution to generate a compact PD-L1 variant with high-affinity PD-1 binding, low immunogenicity risk, and tunable architecture. The DM variant expands the design space of ligand-based checkpoint inhibitors and offers a versatile platform for next-generation immunotherapeutics engineered through synthetic biology frameworks.
Authors
- Ji Yeon Ha
- Seong-Wook Park (ORCID: https://orcid.org/0000-0003-1812-2121)
- Sang Taek Jung (ORCID: https://orcid.org/0000-0001-7217-979X)
- Yong‐Sung Kim (ORCID: https://orcid.org/0000-0003-2673-1509)
- Petrina Jebamani (ORCID: https://orcid.org/0009-0007-6101-6451)
- Sun‐Gu Lee (ORCID: https://orcid.org/0000-0002-3331-1791)
- Dong-Woo Kim (ORCID: https://orcid.org/0000-0002-8099-0715)
Institutions
- Seoul National University (KR)
- Korea University (KR)
- National University (SD)
- National University College (PR)
- Korea University (JP)
- Pusan National University (KR)
- Ajou University (KR)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2025-12-04
- DOI
- https://doi.org/10.1186/s13036-025-00577-x
- Primary Topic
- CAR-T cell therapy research
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Research Foundation
- National Research Foundation of Korea
- Korea Drug Development Fund
- Ministry of Education, India
- Ministry of Science and ICT, South Korea