Bacteria-responsive DNAgel system for targeted delivery of photothermally enhanced MXene/MoS2 in the treatment of pyogenic osteomyelitis
Pyogenic osteomyelitis (POM) presents significant clinical challenges due to persistent infections and impaired bone regeneration. Here, we developed a bacteria-responsive MXene/MoS<sub>2</sub> DNAgel (MXMoS<sub>2</sub> DNAgel) with enhanced photothermal antibacterial capabilities to treat POM. By doping molybdenum disulfide (MoS<sub>2</sub>) quantum dots onto MXene nanosheets, the hydrogel exhibited significantly improved near-infrared (NIR) photothermal conversion, facilitating efficient bacterial eradication. The DNA-based hydrogel responded selectively to bacterial DNase, allowing for infection-triggered release of therapeutic components. Furthermore, the MXMoS<sub>2</sub> DNAgel reduced inflammation by modulating macrophage polarization from pro-inflammatory M1 toward anti-inflammatory M2 phenotypes and promoted osteogenic differentiation through activation of the Wnt signaling pathway. Both <i>in vitro</i> and <i>in vivo</i> studies demonstrated excellent antibacterial efficacy, robust bone regeneration, and favorable biocompatibility. Collectively, this multifunctional hydrogel offers a promising therapeutic platform for targeted management and healing of infectious bone diseases.
Authors
- Jiaxing Gong (ORCID: https://orcid.org/0000-0001-6454-5956)
- Yun Jiang (ORCID: https://orcid.org/0000-0002-1195-1843)
- Mengfei Yu (ORCID: https://orcid.org/0000-0002-7700-4697)
- David Tai Leong (ORCID: https://orcid.org/0000-0001-8539-9062)
- Kuoran Xing (ORCID: https://orcid.org/0009-0002-9682-6829)
- Sitong Lin (ORCID: https://orcid.org/0000-0001-6000-9523)
- Lixuen Siow (ORCID: https://orcid.org/0000-0002-8067-8772)
- Tianyi Gu (ORCID: https://orcid.org/0000-0003-0767-4537)
- Bin Feng (ORCID: https://orcid.org/0000-0002-4040-163X)
- Zhijun Cao (ORCID: https://orcid.org/0000-0002-5519-9462)
- Yunxia Gao (ORCID: https://orcid.org/0000-0003-3707-812X)
- Mingkai Lin (ORCID: https://orcid.org/0000-0003-2610-6658)
- Siyi Zhou (ORCID: https://orcid.org/0000-0002-1022-3828)
- Yu Liu (ORCID: https://orcid.org/0000-0001-6367-9637)
- Yu Yang (ORCID: https://orcid.org/0000-0002-4518-3299)
- Chuhan Zhang (ORCID: https://orcid.org/0000-0002-2897-3918)
- Ziyu Zhu (ORCID: https://orcid.org/0000-0001-7680-5292)
- Romain Lan (ORCID: https://orcid.org/0000-0002-5612-880X)
Institutions
- National University of Singapore (SG)
- Shanghai Jiao Tong University (CN)
- International Peace Maternity & Child Health Hospital (CN)
- Stomatology Hospital (CN)
- Zhejiang University (CN)
Publication Details
- Journal
- Bioactive Materials
- Published
- 2025-12-03
- DOI
- https://doi.org/10.1016/j.bioactmat.2025.10.023
- Primary Topic
- Nanoplatforms for cancer theranostics
- Type
- article
- Field-Weighted Citation Impact
- 0.00