Multiepitope fusion protein-based ELISA for enhanced brucellosis serodiagnosis
When evaluated against a clinically relevant control cohort of non-brucellosis febrile patients, the fusion protein demonstrated a substantial advantage by exhibiting significantly lower cross-reactivity compared to LPS, which frequently cross-reacted with other bacterial infections. The multiepitope fusion protein developed in this study demonstrates significant potential as a diagnostic tool for brucellosis. However, the study's limitations, including a small sample size and lack of information on Brucella species, suggest the need for further research with larger and more diverse sample sets to fully validate its clinical application.
Authors
- Lili Lian
- Tiansong Zhan (ORCID: https://orcid.org/0000-0001-9793-7139)
- Yan Li (ORCID: https://orcid.org/0000-0002-8391-7767)
- Wei Han
- Shuangshuang Li (ORCID: https://orcid.org/0000-0002-5885-3706)
- Dehui Yin (ORCID: https://orcid.org/0000-0002-7164-9320)
- Y. G. Xie (ORCID: https://orcid.org/0009-0006-3489-5879)
Institutions
- Xuzhou Medical College (CN)
- Jilin University (CN)
- General Department of Preventive Medicine (VN)
- First Hospital of Jilin University (CN)
- Heze Municipal Hospital (CN)
- Second Affiliated Hospital of Xuzhou Medical College (CN)
- Shandong Center for Disease Control and Prevention (CN)
Publication Details
- Journal
- PLoS neglected tropical diseases
- Published
- 2025-12-03
- DOI
- https://doi.org/10.1371/journal.pntd.0013804
- Primary Topic
- Brucella: diagnosis, epidemiology, treatment
- Type
- article
- Field-Weighted Citation Impact
- 0.00