Antiproliferative Activity of Prodigiosin Derived From <i>Serratia marcescens</i> VITSD2: An In Vitro and In Silico Approach

The red color pigment prodigiosin is a potent antioxidant produced by different strains of Serratia marcescens and other bacteria. The bio pigment demonstrates many hopeful impending bioactivities. Prodigiosin is an active proapoptotic agent against various cancer cell lines. In the present study, pigment produced from soil isolate Serratia marcescens VITSD2 was characterized and identified using UV, FTIR, GC-MS and NMR analysis (¹H NMR and ¹³C NMR). The antiproliferative activity of prodigiosin pigment from Serratia marcescens VITSD2 was evaluated on cancer cell lines. The active sites and binding patterns of molecular marker survivin was analyzed on docking against prodigiosin.A strong antioxidant potential was noticed at 5 mg/mL concentration with 70 ± 0.08% scavenging activity (2,2-diphenyl-1-picrylhydrazyl)-DPPH. The dose dependent inhibition of HepG2 cell proliferation was observed maximum with 67 ± 0.08% cytotoxic activity at 50 µg/mL. When compared to other cell lines, A549, HL 60 and MCF-7, prodigiosin had a strong inhibitory activity on HepG2 cells. The Rf value of single band obtained in chromatography showed a value of 0.45. Maximum absorbance was observed at 535 nm. The pigment revealed the characteristic functional properties of the prodigiosin. On docking, the lowest binding energy exhibited was found to be -5.15 kcal/mol. The RMSD analysis indicated that the backbone structure converges at 18 ns before it attains stability. Pigment production from Serratia marcescens VIT SD2 offer a renewable and sustainable alternative to synthetic pigments, reducing dependence on nonrenewable resources. The study outcomes specified that the bio pigment prodigiosin extracted from Serratia marcescens VIT SD2 is a promising drug candidate for therapeutics.