Genome sequence data of multidrug-resistant Enterococcus faecalis HMTZ24 carrying multiple virulence factors, isolated from a urinary tract infection in Mosul, Iraq

<i>Enterococcus faecalis</i> is a common pathogen associated with urinary tract infections (UTIs) worldwide. Here we present the draft genome sequence of <i>E. faecalis</i> strain HMTZ24, isolated from the urine of a female patient in Mosul, Iraq. Whole-genome sequencing was performed on the Illumina NovaSeq 6000 platform. The assembled genome is 2,623,745 base pairs (bp) in length distributed across 128 contigs, with an N50 of 41,811 bp and a GC content of 37.73%. Annotation revealed 2,510 coding sequences (CDSs), 50 tRNAs, and 5 rRNA genes. Phylogenomic taxonomy analysis indicated that strain HMTZ24 is closely related to <i>E. faecalis</i> NBRC 100480 (=ATCC 19433) with a digital DNA-DNA hybridisation (dDDH) value of 92.9 % and an average nucleotide identity (ANI) of 99.16 %. Multilocus sequence typing (MLST) assigned the HMTZ24 strain to sequence type 28 (ST28). The genome harbors six antimicrobial resistance genes confirming resistance to nalidixic acid, ciprofloxacin, chloramphenicol, erythromycin, rifampin, trimethoprim, lincomycin, clindamycin, tetracycline, and vancomycin. Two mobile genetic elements (MGEs) including Tn6009 and ISLgar5, and 14 virulence factor genes including <i>ebpA, ebpB, ebpC, ace, strA, espfs, cad, camE, cCf10, cOB1, gelE, tpx, efaA</i>, and <i>ElrA</i> were also identified. The dataset provides a valuable genomic resource for comparative analyses of <i>E. faecalis</i> strains, supporting studies on antimicrobial resistance, virulence factors and regional epidemiology. The draft genome sequence of strain HMTZ24 has been deposited in NCBI under the accession number JBISBO000000000.1.