Controlled-environment platform for electrophysiology recordings on organoids with Neuropixels 2.0
We present a custom recording platform that enables electrophysiological recordings with Neuropixels 2.0 from human brain and heart organoids by maintaining a stable, physiological environment of 5% CO2, 37 °C, and 95% relative humidity. The platform combines a modular positioning system with real-time camera guidance for precise probe alignment. We validated the system by recording consistent bursting activity in brain organoids over a 110-min period and in heart organoids for up to 11 h.
Authors
- Tjitse van der Molen (ORCID: https://orcid.org/0000-0001-6147-542X)
- Julia Sala‐Jarque (ORCID: https://orcid.org/0000-0002-9103-7305)
- Kenneth S. Kosik (ORCID: https://orcid.org/0000-0003-3224-5179)
- Katherine Huang (ORCID: https://orcid.org/0009-0000-7240-6622)
- Carolina M. Camargo (ORCID: https://orcid.org/0000-0002-5918-4081)
- Zongren Zhang (ORCID: https://orcid.org/0009-0001-7709-9980)
- Paul K. Hansma (ORCID: https://orcid.org/0000-0002-0719-0615)
- Mookwang Kwon (ORCID: https://orcid.org/0009-0004-0937-4022)
- Sebastian J. Streichan (ORCID: https://orcid.org/0000-0002-6105-9087)
- Raymond Gifford
- Markus Merk (ORCID: https://orcid.org/0000-0001-6103-7331)
- Kelly Ilmi (ORCID: https://orcid.org/0009-0003-4652-1821)
- Javier j Reveles (ORCID: https://orcid.org/0009-0002-5474-450X)
- Niketh Keshavan (ORCID: https://orcid.org/0009-0001-1862-9347)
- Vrinda Chhatrapati (ORCID: https://orcid.org/0009-0005-7521-5174)
- Sergey Sokolovskiy (ORCID: https://orcid.org/0009-0009-3119-5887)
- Emma Chau (ORCID: https://orcid.org/0009-0005-4486-856X)
Institutions
- University of California, Santa Barbara (US)
- Columbia University (US)
Publication Details
- Journal
- Review of Scientific Instruments
- Published
- 2025-12-01
- DOI
- https://doi.org/10.1063/5.0283114
- Citations
- 1
- Primary Topic
- Neuroscience and Neural Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.91