Development of an Automated Dip‐Type ELISA System

ABSTRACT This study introduces an automated enzyme‐linked immunosorbent assay (ELISA) system employing a novel dip methodology. Unlike traditional ELISA, which requires complex liquid handling, this approach keeps samples and reagents stationary while immersing antibody‐coated reaction fields sequentially. This design eliminates pumps and tubing, reducing maintenance, reagent waste, and overall costs. The system, controlled via a microcontroller and web‐based interface, demonstrated accurate and efficient C‐reactive protein analysis in human serum. With reduced reagent volumes, lower operational costs, and simplified handling, this system offers significant potential for point‐of‐care testing, telemedicine, and broader next‐generation healthcare applications.