A genotype‐independent and highly efficient <i>Agrobacterium</i> ‐mediated soybean genetic transformation system
A stable and efficient transformation system is crucial for functional genomics and trait improvement in soybean. This study developed a tissue culture based genetic transformation system incorporating dual selection (Spectinomycin and RUBY). This system significantly enhances transformation efficiency, shortens the transformation cycle, and demonstrates broad genotype independence, providing a powerful tool for soybean research and breeding.
Authors
- Leping Geng (ORCID: https://orcid.org/0000-0002-3845-4046)
- Hongtao Xie (ORCID: https://orcid.org/0009-0006-0963-6242)
- Jianhua Zhu (ORCID: https://orcid.org/0009-0003-3391-7114)
- Jian‐Kang Zhu (ORCID: https://orcid.org/0000-0001-5134-731X)
- Yanyan Zhang (ORCID: https://orcid.org/0009-0001-8194-4956)
- Fengxue Jing (ORCID: https://orcid.org/0009-0001-0811-3566)
Institutions
- Anhui Agricultural University (CN)
- Southern University of Science and Technology (CN)
- Anhui Academy of Agricultural Sciences (CN)
- Kai Biotech (South Korea) (KR)
Publication Details
- Journal
- Journal of Integrative Plant Biology
- Published
- 2025-12-03
- DOI
- https://doi.org/10.1111/jipb.70102
- Citations
- 1
- Primary Topic
- Plant tissue culture and regeneration
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Natural Science Foundation of China