Recovery of Infectious Oz Virus From Cloned cDNA
Oz virus (OZV) is a tick-borne, six-segmented, negative-strand RNA virus in the genus Thogotovirus, family Orthomyxoviridae. A fatal human infection was reported in Japan in 2023. In this study, we established a reverse genetics system to generate infectious recombinant OZV. Six plasmids encoding the full-length OZV genome segments under the control of a murine RNA polymerase I promoter, together with four plasmids expressing viral proteins essential for polymerase activity, were cotransfected into murine cells. This approach enabled efficient recovery of infectious OZV. The recovered recombinant virus exhibited replication kinetics comparable to wild-type OZV. This system provides a platform for molecular studies of OZV.
Authors
- Yusuke Matsumoto (ORCID: https://orcid.org/0000-0003-3500-2279)
- Lipi Akter (ORCID: https://orcid.org/0000-0002-6317-9410)
- Tofazzal Md Rakib (ORCID: https://orcid.org/0000-0003-2642-9908)
- Shuzo Urata (ORCID: https://orcid.org/0000-0003-0710-2272)
- Hiromichi Matsugo (ORCID: https://orcid.org/0000-0002-4214-5254)
- Yudai Kuroda (ORCID: https://orcid.org/0000-0002-7862-6658)
- Hiroshi Shimoda (ORCID: https://orcid.org/0000-0002-3986-5541)
Institutions
- Kagoshima University (JP)
- University of Nagasaki (JP)
- Yamaguchi University (JP)
- National Institute of Infectious Diseases (JP)
- University of Tokyo Health Sciences (JP)
- Nagasaki Medical Center (JP)
- Nagasaki University (JP)
- Chattogram Veterinary and Animal Sciences University (BD)
- The University of Tokyo (JP)
Publication Details
- Journal
- Microbiology and Immunology
- Published
- 2025-12-03
- DOI
- https://doi.org/10.1111/1348-0421.70029
- Primary Topic
- Viral Infections and Vectors
- Type
- article
- Field-Weighted Citation Impact
- 0.00