Evaluate the Regeneration of Pudendal Nerve Neurotmesis in Rabbit Model

The pudendal nerve (PDN) plays a crucial role in external urethral sphincter (EUS) innervation, contributing to urinary control and coordinating the action of bladder with the external urethral sphincter. This study aims to evaluate the impact of pudendal nerve neurotmesis, with and without subsequent end-to-end coaptation on external urethral sphincter function and coordination with bladder. Twenty healthy female New Zealand White rabbits were randomly divided into two groups. In the control group, pudendal nerve neurotmesis was performed without any subsequent intervention. In the treated group, pudendal nerve neurotmesis was followed by end-to-end nerve coaptation. Clinical evaluation was assessed weekly post-operative (PO) through bladder dairy calculation weekly that include: incontinence episodes, daily fluid intake, volume of each void, and periods between each void, to monitor urination patterns and behavioral changes. Cystometry test in 4th and 16th weeks PO that include: detrusor pressure (P det), bladder capacity (BC), bladder compliance (BCom), leak point pressure (LPP), and frequency (number of leaks) to measure bladder dynamics and physiological responses. The results revealed high significant differences (P ≤ 0.05) between control and treated groups, including alterations in bladder dairy evaluations and in cystometry parameters. In concluded of end-to-end pudendal nerve coaptation promote functioning nerve regeneration which helps restore external urethral sphincter functions.