Impact of Nanocalcium Phosphate on Layers Productivity, Bone Traits, Blood Biochemical Parameters, and Gene Expression

This experiment examined the effects of substituting nano calcium phosphate (NCaP) for conventional dicalcium phosphate (DCP) on laying hen productivity, health, bone traits, gene expression of intestinal and kidney P co-transporters (NaPi-IIa and NaPi-IIb), and gene expression of Ca calbindin in the duodenum and jejunum. At 27 weeks of age, 500 Lohmann Brown laying hens were divided into five equal groups and given different diets. One group received a diet that included 100% DCP (T1), while the other four groups received diets that replaced the DCP with 100, 75, 50, or 25% NCaP, respectively. The highest egg production, weight, and feed consumption were seen in birds fed 50% NCaP (P≤0.05). In contrast, adding NCaP at various amounts outperformed control. Diet groups with NCaP at different doses exhibited thicker, heavier, and more proportional eggshells than the control group (P≤0.05). The best egg and eggshell calcium retention was in 50% NCaP-fed hens. More particularly, NCaP groups increased tibia breaking strength and weight, with 50%NCaP having the highest results. Although NCaP reduced phosphorus excretion by increasing NaPi-IIa expression in the kidney's proximal tubule epithelial cells at their most apical brush edge. The greatest (P≤0.05) gene expression of Calbindin and NaPi-IIb in the duodenum and jejunum was seen in the 50% NCaP group. The inclusion of NCaP improved egg quality and productivity. Gene expressions with calcium and phosphorus retention data show that NCaP-fed birds had higher bioavailability and lower environmental impact. The best was 50% NCaP.