Control of Motile Aeromonas Septicemia (MAS) Disease Using Aeromonas hydrophila GPL-04 Feed-base Vaccine in Gourami Fish (Osphronemus gourami)

The disease that generally attacks gourami fish is Motile Aeromonas Septicemia caused by Aeromonas hydrophila. The use of antibiotics is not recommended because it can cause negative impacts such as environmental pollution, accumulation of residues that are harmful to consumers, and bacterial resistance. A safe and environmentally friendly alternative is to provide vaccination. This study aims to determine the effect of vaccinated feed using Aeromonas hydrophila GPL-04 on immunity in gourami fish. The study employed an experimental laboratory method, utilizing a completely randomized design with four treatments and three repetitions. The treatments given were vaccinated feed with doses of T1 (10 mL 100 g-1 feed for 10 days), T2 (10 mL 100 g-1 feed for 15 days), T3 (15 mL 100 g-1 feed for 10 days), and T4 (15 mL 100 g-1 feed for 15 days) and control (T0). The immunity parameters observed were antibody titer, survival rate (SR), relative percent survival (RPS), and mean time to death (MTD). Observations of immunity parameters were conducted on days 0, 7, 14, 21, and 28 by taking blood through the vena cava caudalis. The results showed that administering feed vaccinated to gourami fish for 28 days of the study could significantly increase antibody titer and SR, but not significantly for the RPS and MTD. The optimum dose of vaccinated feed for gourami fish in this study was 15 mL/100 g-1 feed for 15 days of administration (T4).