Migrasomes in Ischemic Stroke: Molecular Landscape and Pathophysiological Impact
Ischemic stroke (IS) initiates complex systemic responses that extend beyond focal brain injury. To capture these multifaceted changes, proteomics, untargeted metabolomics, and lipidomics are integrated from IS patients spanning a range of clinical severities. This multi-omics approach reveals distinct molecular subtypes characterized by immune activation, oxidative stress, and metabolic dysregulation. Notably, elevated levels of migrasomes are identified in patient plasma and mouse brain tissue. Proteomic profiling of these migrasomes shows enrichment in complement, coagulation, and cholesterol-associated pathways. Functional assays further demonstrate that migrasomes derived from peripheral immune cells exacerbate ischemic injury and intensify post-stroke inflammation. Together, these findings position migrasomes as critical drivers of IS pathophysiology and highlight them as promising targets for therapeutic intervention.
Authors
- Yi Kang
- Yingming Song (ORCID: https://orcid.org/0000-0001-7502-1285)
- Buchang Zhao (ORCID: https://orcid.org/0009-0008-2557-5165)
- Jiehong Yang (ORCID: https://orcid.org/0000-0002-2758-0485)
- Haitong Wan (ORCID: https://orcid.org/0000-0001-5726-7214)
- Qun Hou
- Min Shi
- Haofang Wan (ORCID: https://orcid.org/0000-0001-5055-312X)
- Peng Zhou (ORCID: https://orcid.org/0009-0000-5536-1506)
- Wei Fu (ORCID: https://orcid.org/0000-0001-8155-431X)
- Yingying Zhang (ORCID: https://orcid.org/0000-0001-8794-364X)
- Lingling Li (ORCID: https://orcid.org/0000-0003-3742-8548)
- Chen Ding (ORCID: https://orcid.org/0000-0001-8673-3464)
- Bin Xu (ORCID: https://orcid.org/0000-0002-0027-3039)
- Huifen Zhou
- Yuping Wan
- Hongbo Zhang
Institutions
- Zhejiang Chinese Medical University (CN)
- Fudan University (CN)
- Huzhou Normal University (CN)
- Jining Traditional Chinese Medicine Hospital (CN)
- Luohe Medical College (CN)
- Buchang Pharma (China) (CN)
- The Third Affiliated Hospital of Zhejiang Chinese Medical University (CN)
- Shaanxi Provincial Hospital of Traditional Chinese Medicine (CN)
- Jingzhou Central Hospital (CN)
- First Affiliated Hospital of Henan University (CN)
- First People's Hospital of Chongqing (CN)
- Ningxia Center for Diseases Prevention and Control (CN)
Publication Details
- Journal
- Advanced Science
- Published
- 2025-12-03
- DOI
- https://doi.org/10.1002/advs.202520608
- Primary Topic
- Neuroinflammation and Neurodegeneration Mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- National Natural Science Foundation of China
- Tsinghua University
- National Science and Technology Major Project
- Natural Science Foundation of Zhejiang Province