Lactic Acid Bacteria in Cheese Production

Starter cultures of lactic acid bacteria define the sensory profile of cheese, as well as its microbiological safety and shelf life. Under the current import restrictions, Russia needs efficient domestic starter strains with probiotic properties. Research in this sphere opens up prospects for new functional cheeses that combine conventional sensory properties with health benefits. The authors tested Enterococcus faecium, Lactobacillus acidophilus, L. plantarum, and Weissella thailandensis for starter culture potential. The highest antimicrobial activity against Escherichia coli belonged to L. plantarum (B-11264) and W. thailandensis (B-10412). E. faecium (B-4054) and L. acidophilus (B-2585) demonstrated strong acid-forming effects, which contributed to a decrease in pH. Their resistance to high NaCl concentrations was strain-specific: while 2–4% NaCl inhibited some cultures, 6.5% NaCl was able to inhibit them all. The biocompatibility test revealed no antagonism between the strains, which confirmed good consortia prospects. Strains B-4054, B-2585, and B-11264 demonstrated a reliable enzymatic potential and steady growth under the experimental conditions. In the future, they may provide a strategic alternative to imported probiotic starters. Research prospects involve optimization of cheese production technology by using these strains in microbial consortia.

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Publication Details

Journal
Cheesemaking and buttermaking
Published
2025-11-28
DOI
https://doi.org/10.21603/2073-4018-2025-4-35
Primary Topic
Probiotics and Fermented Foods
Type
article
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article

Lactic Acid Bacteria in Cheese Production

О. А. Неверова, Alexandra Zaushintsena, Vladimir Yustratov, Anna Sergeevna Frolova et al.
Cheesemaking and buttermaking
Probiotics and Fermented Foods
article

Lactic Acid Bacteria in Cheese Production

О. А. Неверова, Alexandra Zaushintsena, Vladimir Yustratov, Anna Sergeevna Frolova, Anna Loseva, Darya Chekushkina, Timothy Larichev
article en

Abstract

Starter cultures of lactic acid bacteria define the sensory profile of cheese, as well as its microbiological safety and shelf life. Under the current import restrictions, Russia needs efficient domestic starter strains with probiotic properties. Research in this sphere opens up prospects for new functional cheeses that combine conventional sensory properties with health benefits. The authors tested Enterococcus faecium, Lactobacillus acidophilus, L. plantarum, and Weissella thailandensis for starter culture potential. The highest antimicrobial activity against Escherichia coli belonged to L. plantarum (B-11264) and W. thailandensis (B-10412). E. faecium (B-4054) and L. acidophilus (B-2585) demonstrated strong acid-forming effects, which contributed to a decrease in pH. Their resistance to high NaCl concentrations was strain-specific: while 2–4% NaCl inhibited some cultures, 6.5% NaCl was able to inhibit them all. The biocompatibility test revealed no antagonism between the strains, which confirmed good consortia prospects. Strains B-4054, B-2585, and B-11264 demonstrated a reliable enzymatic potential and steady growth under the experimental conditions. In the future, they may provide a strategic alternative to imported probiotic starters. Research prospects involve optimization of cheese production technology by using these strains in microbial consortia.

Cheesemaking and buttermaking
Kemerovo State University (RU)
Openalex Percentile: Top 57%
Probiotics and Fermented Foods
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Lactic Acid Bacteria in Cheese Production — О. А. Неверова, Alexandra Zaushintsena, et al. · Cheesemaking and buttermaking (2025) | TGRS Research Map | TGRS