Clinical Study evaluating the Diagnostic Value of Cardiac Ultrasound Parameters Combined With Serum <scp>miR</scp> ‐324‐3p in Septic Cardiac Dysfunction

Ultrasonic examinations showed significant limitations in the early detection and prognostic prediction of sepsis-induced myocardial dysfunction (SIMD), needing assisting biomarkers. The diagnostic and prognostic potential of miR-324-3p in SIMD was evaluated in this study, aiming to explore a novel biomarker. The functional role of miR-324-3p in regulating myocardial cell injury was assessed in H9c2 cells. The study enrolled 210 sepsis patients, including 98 patients diagnosed with SIMD. The significance of miR-324-3p in SIMD risk prediction, screening, and prognosis prediction was evaluated. Moreover, ultrasonic parameters, involving LVEF, LVDd, LAD, and FS, were compared between sepsis and SIMD patients, and the diagnostic and prognostic values of these parameters were also assessed. Silencing miR-324-3p exerted significantly protective effects on myocardial cell injury. Increasing serum miR-324-3p was observed in SIMD patients and was positively correlated with Dallas grades. LVEF, LVDd, LAD, and FS also showed significance in discriminating SIMD patients, but a single parameter cannot possess both satisfactory sensitivity and specificity. Serum miR-324-3p could help improve the diagnostic accuracy, sensitivity, and specificity of ultrasonic parameters in screening SIMD, and miR-324-3p was also identified as an independent risk factor for the adverse prognosis of SIMD patients.