CD47 expression in solid tumors correlates with phagocytic tumor-associated macrophage gene signature
We characterized CD47 expression across key solid tumor indications being evaluated clinically using anti-CD47 blockade agents: HNSCC, breast cancer and CRC. Using a CD47 IHC assay, we identified HNSCC as an indication with the highest CD47 expression. In addition, we quantified tumor macrophages using multiplex immunofluorescence (mIF) and determined that HNSCC had the highest density of TAMs. Compared to relatively low CD47 expression in primary CRC tumors, CRC liver metastases had very high CD47 expression. Quantification of TAM signatures and CD47 expression represent key biomarkers to monitor in patient samples during exploration of CD47-blockade agents in the clinic.
Authors
- Abhishek Aggarwal (ORCID: https://orcid.org/0000-0003-3295-1954)
- Nicholas van Buuren (ORCID: https://orcid.org/0000-0003-2746-4422)
- Azadeh Madjidi
- Juana P. Correa (ORCID: https://orcid.org/0000-0002-2855-2734)
- Kai‐Hui Sun
- Szu-Wen Liu
- Shahed Iqbal
- Jared Odegard (ORCID: https://orcid.org/0009-0005-6516-0026)
- Mengshu Xu (ORCID: https://orcid.org/0000-0001-5329-9235)
- Shiva Zaboli
- Min Wang (ORCID: https://orcid.org/0009-0004-3693-3619)
- Li Li (ORCID: https://orcid.org/0000-0003-2247-5720)
- Ruidong Li (ORCID: https://orcid.org/0000-0002-4842-3681)
- Qiu Zhang (ORCID: https://orcid.org/0000-0003-3079-262X)
- Kelli L. Boyd
- Christina Moon (ORCID: https://orcid.org/0009-0005-3682-2262)
Institutions
- Blackstone (United States) (US)
- Exosome Diagnostics (United States) (US)
- Computational Physics (United States) (US)
- Gilead Sciences (United States) (US)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2025-12-03
- DOI
- https://doi.org/10.3389/fimmu.2025.1699237
- Primary Topic
- Phagocytosis and Immune Regulation
- Type
- article
- Field-Weighted Citation Impact
- 0.00