Effect of <i>Streptomyces pylosus</i> (LMA1) cell free extract on the staphylococcal-infected cell lines

Objectives: The experimentation on cell lines is a prerequisite as well as an optimise method for the screening of natural drugs. Materials and Methods: The cells with 70% confluency were selected for the viability in the presence and absence of the test metabolite extracted from Laboratory of Microbiology Actinomycetes 1 (LMA1) ( Streptomyces pilosus ), which was considered for the screening as an ‘anti-infective agent’ using the cell line culture. Results: It reveals that the cells present were observed to be at the stage of stationary as well as death or decline period, indicated by the deformity of the cells and coated with some molecules, inferring the phase of senescence. Conclusion: The experiment carried out had made an attempt to decipher the mode of action of the test actinomycetal LMA1, cell free extract on the cell line culture infected with test bacteria, namely Staphylococcus aureus (BMS4).