Screening strategy to identify Cas9 variants with higher HDR activity based on diphtheria toxin
We explored the possibility of obtaining Cas9 mutants with high HDR efficiency via this screening system.
Authors
- Yu Sato (ORCID: https://orcid.org/0000-0003-4538-4303)
- Wataru Nomura (ORCID: https://orcid.org/0000-0001-8348-7544)
- Daisuke Matsumoto (ORCID: https://orcid.org/0000-0003-3252-7344)
- Tomoko Kato-Inui
- Kosei Kubota (ORCID: https://orcid.org/0000-0003-2408-7545)
- Kiyomi Nigorikawa (ORCID: https://orcid.org/0000-0002-4285-9695)
- Yuichiro Miyaoka
Institutions
- Hiroshima University (JP)
- Yamaguchi University (JP)
- Tokyo Metropolitan Institute of Medical Science (JP)
Publication Details
- Journal
- Journal of Biomedical Science
- Published
- 2025-12-03
- DOI
- https://doi.org/10.1186/s12929-025-01197-9
- Primary Topic
- Complement system in diseases
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Suzuken Memorial Foundation
- Ministry of Education, Culture, Sports, Science and Technology
- Japan Society for the Promotion of Science