PRODUCTION OF RECOMBINANT β-GALACTOSIDASE PROTEIN IN BOMBYX MORI (BMN1) BACULOVIRUS INSECT CELLS

In this study we aimed to obtain a novel recombinant β-galactosidase baculovirus using the Bombyx mori baculovirus – insect cell expression system. A new recombinant plasmid was constructed on the basis of the pBacPAK8 transfer vector and cloned in E. coli cells. Subsequently, a recombinant baculovirus was generated in Bombyx mori cells, and efficient expression of the β-galactosidase gene was observed. The enzymatic activity of the protein was confirmed by the ONPG assay. The results demonstrated that the baculovirus – insect cell system expands the possibilities for producing proteins of industrial significance.