Enamel matrix derivative-induced cementoblast differentiation via Wnt/β-catenin signaling in human periodontal ligament cells

EMD upregulated the expression of CEMP1 and cementum attachment protein. This effect was attenuated by IWP-2, indicating Wnt/β-catenin involvement. A T-cell factor binding site was identified within the CEMP1 promoter. EMD increased the expression of osterix and runt-related transcription factor 2 (Runx2) slightly, but decreased the levels of osteocalcin and osteopontin. No mineralization enhancement was observed based on alkaline phosphatase activity and Alizarin Red S stainingConclusion: EMD promotes early-stage cementoblast differentiation via Wnt/β-catenin signaling but has limited effects on late-stage maturation, offering new insights into the regenerative potential of EMD for periodontal therapy.